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Videoconferencing usage has surged in recent years, but current platforms present significant accessibility barriers for the 430 million d/Deaf or hard of hearing people worldwide. Informed by prior work examining accessibility barriers in current videoconferencing platforms, we designed and developed Jod, a videoconferencing platform to facilitate communication in mixed hearing groups. Key features include support for customizing visual layouts and a notification system to request attention and influence behavior. Using Jod, we conducted six mixed hearing group sessions with 34 participants, including 18 d/Deaf or hard of hearing participants, 10 hearing participants, and 6 sign language interpreters. We found participants engaged in visual layout rearrangements based on their hearing ability and dynamically adapted to the changing group communication context, and that notifications were useful but raised a need for designs to cause fewer interruptions. We provide insights for future videoconferencing designs and conclude with recommendations for conducting mixed hearing studies. 

Abstract Studies using 16S rRNA and shotgun metagenomics typically yield different results, usually attributed to PCR amplification biases. We introduce Greengenes2, a reference tree that unifies genomic and 16S rRNA databases in a consistent, integrated resource. By inserting sequences into a whole-genome phylogeny, we show that 16S rRNA and shotgun metagenomic data generated from the same samples agree in principal coordinates space, taxonomy and phenotype effect size when analyzed with the same tree. 

Abstract Mitochondria house evolutionarily conserved pathways of carbon and nitrogen metabolism that drive cellular energy production. Mitochondrial bioenergetics is regulated by calcium uptake through the mitochondrial calcium uniporter (MCU), a multi-protein complex whose assembly in the inner mitochondrial membrane is facilitated by the scaffold factor MCUR1. Intriguingly, many fungi that lack MCU contain MCUR1 homologs, suggesting alternate functions. Herein, we characterizeSaccharomyces cerevisiaehomologs Put6 and Put7 of MCUR1 as regulators of mitochondrial proline metabolism. Put6 and Put7 are tethered to the inner mitochondrial membrane in a large hetero-oligomeric complex, whose abundance is regulated by proline. Loss of this complex perturbs mitochondrial proline homeostasis and cellular redox balance. Yeast cells lacking either Put6 or Put7 exhibit a pronounced defect in proline utilization, which can be corrected by the heterologous expression of human MCUR1. Our work uncovers an unexpected role of MCUR1 homologs in mitochondrial proline metabolism.